Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
LMNA

Cell type

Cell type Class
Adipocyte
Cell type
Adipose stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Adipose
cell type
Human primary adipose stem cells
tissue
Adipose
time
Day 0
chip antibody
LMNA/C (Santa Cruz sc-7292)
replicate
Rep2

Sequenced DNA Library

library_name
GSM6857884
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP, cells were sonicated to produce chromatin fragments of 200 base-pairs, lysates were clarified by sedimentation and histone-DNA or lamin-DNA complexes were isolated from supernatants by ChIP using specific antibodies. RNA was purified from cell lysates using the RNEasy mini kit (Qiagen). ChIP sequencing libraries were prepared according to Illumina protocols for sequencing at the Norwegian Sequencing Center.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
85109176
Reads aligned (%)
98.3
Duplicates removed (%)
73.5
Number of peaks
1739 (qval < 1E-05)

hg19

Number of total reads
85109176
Reads aligned (%)
97.3
Duplicates removed (%)
75.8
Number of peaks
1312 (qval < 1E-05)

Base call quality data from DBCLS SRA